Riboflavin Test Validation

Riboflavin testing is an evaluation method to ensure spray device(s) effectively apply cleaning solutions for clean in place (CIP) of process vessels. This procedure is only a spray device coverage test and should not be considered a test of vessel cleanability.

Following the final rinse and drain time, vessel surfaces are inspected using a portable UV-A light source. Any riboflavin residues noted by appearance of a yellow-green fluorescence are indicative of a potential spray device failure to adequately rinse riboflavin from that surface.